Invasion and Interaction Studies of Salmonella typhimurium Sub Sp Enteritis in Vero and MDCK Cell Lines

Vero cells and Madin Darby Canin Kidney cells were exposed to Salmonella typhimurium sub spp. enteritis to study the interaction and invasion. The monolayer cell line bottles were selected and sub cultured. The sub cultured bottles were incubated at 37°C in 5% CO atmosphere for the formation of monolayer. 2 After the complete confluence of cells, the bottles were selected for the inoculation of bacteria. The mid logarithmic phase bacterial culture (10 CFU/ml) was used. These infected cell monolayer were incubated at 37°C 5 in 5 % CO environment. After half an hour of bacterial inoculation, the monolayer was observed under the 2 inverted microscope. The shape of the cells became changed. Before inoculation, the shape of the Vero cells was sickle cell shape. The cells become round shape. After 1 hour of bacterial inoculation, the cells were fully changed into round shape and the cells became swollen. The experiment was performed to test the invasion ability of Salmonella on Vero cells and Madin Darby Canin Kidney cells.